FAST rapidly detects and tracks sperm imaged in negative phase microscopy, providing flagellar waveforms, wavelength, beat frequency parameters and measurements of the mean hydrodynamic power dissipation along the flagellum. To save you time in your analysis, FAST also calculates the traditional CASA measures.
FAST is not designed to analyse raw semen, it is speciﬁcally for precise analysis of ﬂagellar kinematics, as that is the promising area for computer use.
Combining FAST with fluid mechanical codes and mathematical models provides for the first time the ability to gain insight into experimentally intractable interactions. By supplementing tracked flagellar beat patterns with fluid mechanics analysis we can understand details of the sperm-generated local flow field, forces and energy dissipation due to flagellar motion in different environments.
FAST has a simple interface and is available on Windows 10, Mac OS X, and Linux. No other package can provide the detailed, high-throughput analysis of beating flagella, with an easy to use interface for rapid analysis of your data.